Effects of docosahexaenoic acid or arachidonic acid supplementation on the behavior of cardiomyocytes derived from human pluripotent stem cells

Background: Human heart changes its energetic substrates from lactate and glucose to fatty acids during the neonatal period. Noticing lack of in media for culture cardiomyocytes derived human pluripotent stem cells (hiPS-CM), researchers have supplemented mixtures hiPS-CM reported enhancement maturation hiPS-CM. In our previous studies, we separately two polyunsaturated (PUFAs), docosahexaenoic acid (DHA) or arachidonic (AA), rat fetal found that supplementations upregulated expressions mRNAs cardiomyocyte differentiation, metabolism, cellular adhesion. The contractility was attributed improvement intercellular connection rather than a direct contractile force. Methods: This study reports successive results effects DHA AA supplementation on addition force mRNA measurements used study, further investigated effect different aggregations output by means finite element analysis, measured metabolites, assessed cTNT MLC2v through immunofluorecsence evaluation. Results: It showed sole albumin-conjugated can be taken up without other uptake-enhancing factors, may activate CD36_­ERRγ metabolic pathway. increased ratio collagen gels stimulated aggregation form clusters. modest since increase not significant. more effective because it significantly P300 CD36. However, analysis formation clusters gel attenuated exerted surroundings. Conclusion: AA, as having been infant formulas, no significant performance when they were individually, although able enter system. some auxiliary hiPS-CM, which is worthy investigation under consideration membrane composition alteration remodeling molecules.